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flag tagged destination vectors  (Genechem)

 
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    Genechem flag tagged destination vectors
    Flag Tagged Destination Vectors, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flag-tagged+destination+vectors/pmc12781079-114-29-33?v=Genechem
    Average 86 stars, based on 1 article reviews
    flag tagged destination vectors - by Bioz Stars, 2026-07
    86/100 stars

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    Figure 2. IP6K1 binds pyrophosphorylation substrate proteins and CK2 (A–C) Co-precipitation of endogenous NOLC1 (A), TCOF (B) or UBF1 (C) with IP6K1. N-terminally <t>SFB-tagged</t> IP6K1 was expressed in HEK293T cells, pulled down with <t>streptavidin-sepharose</t> beads, and probed to detect endogenous NOLC1, TCOF or UBF1. (D)
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    Figure 2. IP6K1 binds pyrophosphorylation substrate proteins and CK2 (A–C) Co-precipitation of endogenous NOLC1 (A), TCOF (B) or UBF1 (C) with IP6K1. N-terminally SFB-tagged IP6K1 was expressed in HEK293T cells, pulled down with streptavidin-sepharose beads, and probed to detect endogenous NOLC1, TCOF or UBF1. (D)

    Journal: Bioscience Reports

    Article Title: Interaction with IP6K1 supports pyrophosphorylation of substrate proteins by the inositol pyrophosphate 5-InsP7

    doi: 10.1042/bsr20240792

    Figure Lengend Snippet: Figure 2. IP6K1 binds pyrophosphorylation substrate proteins and CK2 (A–C) Co-precipitation of endogenous NOLC1 (A), TCOF (B) or UBF1 (C) with IP6K1. N-terminally SFB-tagged IP6K1 was expressed in HEK293T cells, pulled down with streptavidin-sepharose beads, and probed to detect endogenous NOLC1, TCOF or UBF1. (D)

    Article Snippet: Human IP6K1 cDNA (GenBank ID NM 001242829.2) and catalytically inactive IP6K1 (K226A) mutant were subcloned in pCDNA3.1 as previously described [22]. cDNA encoding human IP6K1 domains, i.e., FL, N-lobe, C-lobe, IDR-1, IDR-2, PDKG motif with IP helices, and IDR-1 with C-lobe, were cloned into the N-terminal SFB (S protein, Flag, streptavidin binding peptide)-tag destination vector, using the Gateway cloning strategy (Thermo Fisher Scientific).

    Techniques: